Our lines

Publications

  • Baptista D, Tahmasebi Birgani Z, Widowski H, Passanha F, Stylianidis V, Knoops K, Gubbins E, Iriondo C, Skarp KP, Rottier RJ, Wolfs TG, van Blitterswijk C, LaPointe V, Habibović P, Reynaert NL, Giselbrecht S, Truckenmüller R. Polymer film-based microwell array platform for long-term culture and research of human bronchial organoids. Mater Today Bio. 2023 Mar 14;19:100603. https://doi.org/10.1016/j.mtbio.2023.100603.
  • Carvalho DJ, Kip AM, Romitti M, Nazzari M, Tegel A, Stich M, Krause C, Caiment F, Costagliola S, Moroni L, Giselbrecht S. Thyroid-on-a-chip: An Organoid Platform For In Vitro Assessment of Endocrine Disruption. Adv Healthc Mater. 2022 Dec 22:e2201555. https://doi.org/10.1002/adhm.202201555.
  • Kakni P, Truckenmüller R, Habibović P, van Griensven M, Giselbrecht S. A Microwell-Based Intestinal Organoid-Macrophage Co-Culture System to Study Intestinal Inflammation. Int J Mol Sci. 2022 Dec 6;23(23):15364. https://doi.org/10.3390/ijms232315364.
  • Cox B, Barton P, Class R, Coxhead H, Delatour C, Gillent E, Henshall J, Isin EM, King L, Valentin J-P. Setup of human liver-chips integrating 3D models, microwells and a standardized microfluidic platform as proof-of-concept study to support drug evaluation. Biomater Biosyst. 2022;7:100054. https://doi.org/10.1016/j.bbiosy.2022.100054
  • Fois MG, Tahmasebi Birgani ZN, Guttenplan APM, van Blitterswijk CA, Giselbrecht S, Habibović P, Truckenmüller RK. Assessment of Cell-Material Interactions in Three Dimensions through Dispersed Coaggregation of Microsized Biomaterials into Tissue Spheroids. Small. 2022 Jul;18(29):e2202112. https://doi.org/10.1002/smll.202202112.
  • Baptista D, Moreira Teixeira L, Barata D, Tahmasebi Birgani Z, King J, van Riet S, Pasman T, Poot AA, Stamatialis D, Rottier RJ, Hiemstra PS, Carlier A, van Blitterswijk C, Habibović P, Giselbrecht S, Truckenmüller R. 3D Lung-on-Chip Model Based on Biomimetically Microcurved Culture Membranes. ACS Biomater Sci Eng. 2022 Jun 13;8(6):2684-2699. https://doi.org/10.1021/acsbiomaterials.1c01463.
  • Kakni P, López-Iglesias C, Truckenmüller R, Habibović P, Giselbrecht S. Reversing Epithelial Polarity in Pluripotent Stem Cell-Derived Intestinal Organoids. Front Bioeng Biotechnol. 2022 Apr 25;10:879024. https://doi.org/10.3389/fbioe.2022.879024.
  • Sthijns MMJPE, Rademakers T, Oosterveer J, Geuens T, van Blitterswijk CA, LaPointe VLS. The response of three-dimensional pancreatic alpha and beta cell co-cultures to oxidative stress. PLoS One. 2022 Mar 15;17(3):e0257578. https://doi.org/10.1371/journal.pone.0257578.
  • Samal P, Gubbins E, van Blitterswijk C, Truckenmüller R, Giselbrecht S. Thin fluorinated polymer film microcavity arrays for 3D cell culture and label-free automated feature extraction. Biomater Sci. 2021 Nov 23;9(23):7838-7850. https://doi.org/10.1039/d1bm00718a.
  • Baptista D, Teixeira LM, Birgani ZT, van Riet S, Pasman T, Poot A, Stamatialis D, Rottier RJ, Hiemstra PS, Habibović P, van Blitterswijk C, Giselbrecht S, Truckenmüller R. 3D alveolar in vitro model based on epithelialized biomimetically curved culture membranes. Biomaterials. 2021 Jan;266:120436. https://doi.org/10.1016/j.biomaterials.2020.120436.
  • Kakni P, Hueber R, Knoops K, López-Iglesias C, Truckenmüller R, Habibovic P, Giselbrecht S. Intestinal Organoid Culture in Polymer Film-Based Microwell Arrays. Adv Biosyst. 2020 Oct;4(10):e2000126. https://doi.org/10.1002/adbi.202000126.
  • Keller F, Bruch R, Schneider R, Meier-Hubberten J, Hafner M, Rudolf R. A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro. Cells. 2020 Aug 13;9(8):1900. https://doi.org/10.3390/cells9081900.
  • Kleimaier D, Schepkin V, Nies C, Gottwald E, Schad LR. Intracellular Sodium Changes in Cancer Cells Using a Microcavity Array-Based Bioreactor System and Sodium Triple-Quantum MR Signal. Processes. 2020;8(10):1267. https://doi.org/10.3390/pr8101267.
  • Kleimaier D, Goerke S, Nies C, Zaiss M, Kunz P, Bachert P, Ladd ME, Gottwald E, Schad LR. The cellular heat shock response monitored by chemical exchange saturation transfer MRI. Sci Rep. 2020 Jul 6;10(1):11118. https://doi.org/10.1038/s41598-020-68022-1.
  • Samal P, Maurer P, van Blitterswijk C, Truckenmüller R, Giselbrecht S. A New Microengineered Platform for 4D Tracking of Single Cells in a Stem-Cell-Based In Vitro Morphogenesis Model. Adv Mater. 2020 Jun;32(24):e1907966. https://doi.org/10.1002/adma.201907966.
  • von Molitor E, Riedel K, Hafner M, Rudolf R, Cesetti T. Sensing Senses: Optical Biosensors to Study Gustation. Sensors (Basel). 2020 Mar 25;20(7):1811. https://doi.org/10.3390/s20071811.
  • Nürnberg E, Vitacolonna M, Klicks J, von Molitor E, Cesetti T, Keller F, Bruch R, Ertongur-Fauth T, Riedel K, Scholz P, Lau T, Schneider R, Meier J, Hafner M, Rudolf R. Routine Optical Clearing of 3D-Cell Cultures: Simplicity Forward. Front Mol Biosci. 2020 Feb 21;7:20. https://doi.org/10.3389/fmolb.2020.00020.
  • Hoesl MAU, Kleimaier D, Hu R, Malzacher M, Nies C, Gottwald E, Schad LR. 23 Na Triple-quantum signal of in vitro human liver cells, liposomes, and nanoparticles: Cell viability assessment vs. separation of intra- and extracellular signal. J Magn Reson Imaging. 2019 Aug;50(2):435-444. https://doi.org/10.1002/jmri.26666.
  • Nies C, Rubner T, Lorig H, Colditz V, Seelmann H, Müller A, Gottwald E. A Microcavity Array-Based 4D Cell Culture Platform. Bioengineering (Basel). 2019 May 31;6(2):50. https://doi.org/10.3390/bioengineering6020050.
  • Gottwald E, Nies C, Wuchter P, Saffrich R, Truckenmüller R, Giselbrecht S. A Microcavity Array-Based 3D Model System of the Hematopoietic Stem Cell Niche. Methods Mol Biol. 2019;2017:85-95. https://doi.org/10.1007/978-1-4939-9574-5_7.
  • Christoffersson J, Meier F, Kempf H, Schwanke K, Coffee M, Beilmann M, Zweigerdt R, Mandenius CF. A Cardiac Cell Outgrowth Assay for Evaluating Drug Compounds Using a Cardiac Spheroid-on-a-Chip Device. Bioengineering (Basel). 2018 May 4;5(2):36. https://doi/10.3390/bioengineering5020036.
  • Schneider AK, Nikolov PM, Giselbrecht S, Niemeyer CM. DNA-SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells. Small. 2017 May;13(17):1603923. https://doi.org/10.1002/smll.201603923.
  • Wuchter P, Saffrich R, Giselbrecht S, Nies C, Lorig H, Kolb S, Ho AD, Gottwald E. Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells. Cell Tissue Res. 2016 Jun;364(3):573-584. https://doi.org/10.1007/s00441-015-2348-8.
  • Vrij EJ, Espinoza S, Heilig M, Kolew A, Schneider M, van Blitterswijk CA, Truckenmüller RK, Rivron NC. 3D high throughput screening and profiling of embryoid bodies in thermoformed microwell plates. Lab Chip. 2016 Feb 21;16(4):734-742. https://doi.org/10.1039/c5lc01499a.
  • Hirschbiel AF, Geyer S, Yameen B, Welle A, Nikolov P, Giselbrecht S, Scholpp S, Delaittre G, Barner-Kowollik C. Photolithographic patterning of 3D-formed polycarbonate films for targeted cell guiding. Adv Mater. 2015 Apr 24;27(16):2621-2626. https://doi.org/10.1002/adma.201500426.
  • Waterkotte B, Bally F, Nikolov PM, Waldbaur A, Rapp BE, Truckenmueller R, Lahann J, Schmitz K, Giselbrecht S. Biofunctional Micropatterning of Thermoformed 3D Substrates. Adv Funct Mater. 2014;24(4):442-450. https://doi.org/10.1002/adfm.201301093.
  • Truckenmüller R, Giselbrecht S, Escalante-Marun M, Groenendijk M, Papenburg B, Rivron N, Unadkat H, Saile V, Subramaniam V, van den Berg A, van Blitterswijk C, Wessling M, de Boer J, Stamatialis D. Fabrication of cell container arrays with overlaid surface topographies. Biomed Microdevices. 2012 Feb;14(1):95-107. https://doi.org/10.1007/s10544-011-9588-5.
  • Hebeiss I, Truckenmüller R, Giselbrecht S, Schepers U. Novel three-dimensional Boyden chamber system for studying transendothelial transport. Lab Chip. 2012 Feb 21;12(4):829-834. https://doi.org/10.1039/c2lc20733h.
  • Giselbrecht S, Reinhardt M, Mappes T, Börner M, Gottwald E, van Blitterswijk C, Saile V, Truckenmüller R. Closer to nature-bio-inspired patterns by transforming latent lithographic images. Adv Mater. 2011 Nov 9;23(42):4873-4879. https://doi.org/10.1002/adma.201102759.
  • Truckenmüller R, Giselbrecht S, Rivron N, Gottwald E, Saile V, van den Berg A, Wessling M, van Blitterswijk C. Thermoforming of film-based biomedical microdevices. Adv Mater. 2011 Mar 18;23(11):1311-1329. https://doi.org/10.1002/adma.201003538.
  • Richter C, Reinhardt M, Giselbrecht S, Leisen D, Trouillet V, Truckenmüller R, Blau A, Ziegler C, Welle A. Spatially controlled cell adhesion on three-dimensional substrates. Biomed Microdevices. 2010 Oct;12(5):787-795. https://doi.org/10.1007/s10544-010-9433-2.
  • Heilig M, Giselbrecht S, Guber A, Worgull M. Microthermoforming of nanostructured polymer films: a new bonding method for the integration of nanostructures in 3-dimensional cavities. Microsyst Technol. 2010;16:1221-1231. https://doi.org/10.1007/s00542-010-1072-2.
  • Gottwald E, Lahni B, Thiele D, Giselbrecht S, Welle A, Weibezahn KF. Chip-based three-dimensional cell culture in perfused micro-bioreactors. J Vis Exp. 2008 May 21;(15):564. https://doi.org/10.3791/564.
  • Giselbrecht S, Gottwald E, Truckenmueller R, Trautmann C, Welle A, Guber A, Saile V, Gietzelt T, Weibezahn KF. Microfabrication of chip-sized scaffolds for three-dimensional cell cultivation. J Vis Exp. 2008 May 12;(15):699. https://doi.org/10.3791/699.
  • Truckenmüller R, Giselbrecht S, van Blitterswijk C, Dambrowsky N, Gottwald E, Mappes T, Rolletschek A, Saile V, Trautmann C, Weibezahn KF, Welle A. Flexible fluidic microchips based on thermoformed and locally modified thin polymer films. Lab Chip. 2008 Sep;8(9):1570-1579. https://doi.org/10.1039/b803619e.
  • Gottwald E, Giselbrecht S, Augspurger C, Lahni B, Dambrowsky N, Truckenmüller R, Piotter V, Gietzelt T, Wendt O, Pfleging W, Welle A, Rolletschek A, Wobus AM, Weibezahn KF. A chip-based platform for the in vitro generation of tissues in three-dimensional organization. Lab Chip. 2007 Jun;7(6):777-785. https://doi.org/10.1039/b618488j.
  • Heckele M, Guber AE, Truckenmüller R. Replication and bonding techniques for integrated microfluidic systems. Microsyst Technol. 2006;12:1031-1035. https://doi.org/10.1007/s00542-006-0158-3.
  • Giselbrecht S, Gietzelt T, Gottwald E, Trautmann C, Truckenmüller R, Weibezahn KF, Welle A. 3D tissue culture substrates produced by microthermoforming of pre-processed polymer films. Biomed Microdevices. 2006 Sep;8(3):191-199. https://doi.org/10.1007/s10544-006-8174-8.
  • Giselbrecht S, Gietzelt T, Gottwald E, Guber AE, Trautmann C, Truckenmüller R, Weibezahn KF. Microthermoforming as a novel technique for manufacturing scaffolds in tissue engineering (CellChips). IEE Proc Nanobiotechnol. 2004 Aug;151(4):151-157. https://doi.org/10.1049/ip-nbt:20040824.
  • Truckenmüller R, Giselbrecht S. Microthermoforming of flexible, not-buried hollow microstructures for chip-based life sciences applications. IEE Proc Nanobiotechnol. 2004 Aug;151(4):163-166. https://doi.org/10.1049/ip-nbt:20040823.
  • Truckenmüller R, Rummler Z, Schaller T, Schomburg WK. Low-cost thermoforming of micro fluidic analysis chips. J Micromech Microeng. 2002;12(4):375. https://doi.org/10.1088/0960-1317/12/4/304.